Hyaluronan synthesis by epiphysial chondrocytes is regulated by growth hormone, insulin-like growth factor-1, parathyroid hormone and transforming growth factor-β1

摘要: In a previous study, we presented evidence that the synthesis of hyaluronan by hypertrophic chondrocytes is one principal factors driving interstitial expansion growth plate (Pavasant et al., J. Cell Sci. 109: 327–334. 1996). To test this possibility further, used two different approaches to examine effects hormones on production in plate. first approach, examined lit/lit mouse lacks hormone and found its lacunae were smaller contained less than those wild type mice. Moreover, ratios staining density total area similar for wt/wt mice, indicating amount directly related lacuna size. second segments epiphysial placed organ culture. Under normal culture conditions, band progressed across length plate, reflecting maturation into stage. When insulin-like factor-1, factor known promote chondrocyte maturation, was added medium, enlargement stimulated. contrast, when either parathyroid or transforming factor-β1, both which inhibit differentiation, medium cultured segments, new pericellular not detected did enlarge. Taken together, these results indicate stimulate have corresponding effect hyaluronan. This, turn, further supports importance process enlargement.