Effects of platelet lysates on select bone cell functions

摘要: Although platelet-rich plasma and platelet concentrates have been used to promote bone healing in orthopaedic maxillofacial surgery, the underlying cellular-level mechanisms remain poorly understood. The present vitro study investigated effects of human lysate (PL) on selected functions cultured cells. Cells from 18-day-old fetal rat calvaria were isolated by a collagenase digestion procedure. PL was added at different concentrations pre- or post-confluent cell stage. After 1 day, proliferation maximal half-maximal presence 3 x 10(8) 0.5 platelets/ml, respectively. During 17 h, number cells traversing scrape border wound model increased 16-fold platelets/ml. platelets/ml pre-confluent cultures for 48 h resulted threefold decrease alkaline phosphatase (ALP) specific activity. In case confluent cells, (from 10(6) platelets/ml) 11 days, ALP activity total calcium content decreased dose-dependent manner reached minimum summary, short-term exposure (up 24 h) promotes proliferative chemotactic while long-term results both mineral formation. These data show that soluble components contained may affect process modulating differently functions.