Early cell adhesion events differ between osteoporotic and non-osteoporotic osteoblasts.

摘要: In osteoporosis, the regenerative capacity of bone is compromised, which may involve altered osteoblast (OB) activity. This could be attributed to an inappropriate synthesis and assembly extracellular matrix (ECM), cell adhesion ECM, or due downstream activation adhesion-mediated signaling cascades through proteins such as focal kinase (FAK). The purpose our study was compare early events using previously described characterized clinically derived OBs obtained from human patients undergoing major joint arthroplasty for osteoporosis osteoarthritis. presence absence established with a radiographic index. Using light microscopy crystal violet staining, we show that OB cells sites do not attach spread well non-osteoporotic (OP) cells. OP initially have more rounded morphology, significantly less (P<0.001) attachment serum-coated tissue culture plastic over 24 h time period. Immunofluorescent labeling after showed adhesions (FAs) stress fibers were defined, smaller had motile phenotype. When normalized protein lysates Western blotted phosphotyrosine (PY) band corresponding pp125FAK identified. FAK tyrosine phosphorylation evident at 6 in both non-OP OBs, but decreased absent OBs. These results suggest events, adhesion, attachment, via PY