Primary human bone cultures from older patients do not respond at continuum levels of in vivo strain magnitudes.

摘要: Abstract Osteoporosis is characterized by excessive loss of bone mass, while exercise believed to maintain or enhance mass. Since marginally affects osteoporosis, we wondered whether cells from osteoporotic patients would fail respond strain. Primary human bone-like cultures were obtained females over age 60 with hip arthroplasty procedures performed for either fracture ( n =8) non-osteoporotic osteoarthrosis =5). Cultures (96,000 cell/cm 2 ) strained in rectangular optically clear silastic wells. Three periods uniaxial substratum strain (1000 μ-strain, 1 Hz, 10,000 cycles, sine wave) provided every 24 h using a four-point bending, computer-controlled device. Results at frequency 1 Hz compared exposed 20 Hz derived one osteoarthritic subject. Alterations protein level expression bone-related proteins determined semi-quantitative confocal approach along enzyme (alkaline phosphatase) activity and mRNA copy number cRNA RT-PCR. Strain did not alter levels levels, activity, steady state per cell response group. Strained exhibited little variation unstrained controls, individual increases the other. The results suggest that older individuals may be responsive continuum anticipated vigorous activity.