Cloning of novel trinucleotide-repeat (CAG) containing genes in mouse brain
作者： Sun Jung Kim , Bo Hwa Shon , Joo Hyun Kang , Kyung-Soo Hahm , Ook Joon Yoo
关键词: genomic DNA 、 Gene 、 Trinucleotide repeat expansion 、 Molecular biology 、 GenBank 、 Biology 、 Molecular cloning 、 Southern blot 、 cDNA library 、 Homology (biology) 、 Genetics
摘要: Abstract CAG trinucleotide repeat (CTR) sequence often appears in mammalian genome including transcription-regulatory protein and homeobox genes. Its expansion is associated with six genetic disorders human. To identify novel CTR-containing genes expressed mouse brain, a brain cDNA library was screened using an oligonucleotide, (CTG) 10 . Eight clones were they sequenced on both strands. The size of the cloned DNA ranged from 0.5 to 2.1 kb. number repeats 6 25. inserts analyzed for open reading frames peptide sequences used GenBank homology search. Of clones, one (CAG-6) shared 13 consecutive identical amino acid residues OB-cadherin gene, member cadherin family. CAG-14 showed high (657 nucleotides identity 1022 nucleotides; 64%) 3′-untranslated region rat leukocyte common antigen-related (LAR) tyrosine phosphatase receptor. All 8 originated as judged by Southern blot analysis genomic DNA. expression addressed RT-PCR 4 specific expression.
core.ac.uk
kribb.re.kr
elsevier.com参考文章(25)
S S Sommer, D P Jacobson, P Schmeling, Characterization of the patterns of polymorphism in a "cryptic repeat" reveals a novel type of hypervariable sequence. American Journal of Human Genetics. ,vol. 53, pp. 443- 450 ,(1993)
D Ha-Hao, A Lunkes, A Brice, N Santos, T Ratzlaff, L Heredero, I Torrens, G Orozco, J Aguiar, S Gispert, Localization of the candidate gene D-amino acid oxidase outside the refined I-cM region of spinocerebellar ataxia 2. American Journal of Human Genetics. ,vol. 57, pp. 972- 975 ,(1995)
R Williamson, S Chamberlain, J Weissenbach, B S Norte, R Twells, R Allotey, M Pook, C Cemal, The spinocerebellar ataxia 2 locus is located within a 3-cm interval on chromosome 12q23-24.1 American Journal of Human Genetics. ,vol. 57, pp. 185- 189 ,(1995)
Shi-Hua Li, Melvin G. McInnis, Russell L. Margolis, Stylianos E. Antonarakis, Christopher A. Ross, Novel Triplet Repeat Containing Genes in Human Brain: Cloning, Expression, and Length Polymorphisms Genomics. ,vol. 16, pp. 572- 579 ,(1993) , 10.1006/GENO.1993.1232
Stefan-M. Pulst, Alex Nechiporuk, Tamilla Nechiporuk, Suzana Gispert, Xiao-Ning Chen, Iscia Lopes-Cendes, Susan Pearlman, Sidney Starkman, Guillermo Orozco-Diaz, Astrid Lunkes, Pieter DeJong, Guy A. Rouleau, Georg Auburger, Julie R. Korenberg, Carla Figueroa, Soodabeh Sahba, Moderate expansion of a normally biallelic trinucleotide repeat in spinocerebellar ataxia type 2 Nature Genetics. ,vol. 14, pp. 269- 276 ,(1996) , 10.1038/NG1196-269
Jocelyn Laporte, Ling Jia Hu, Christine Kretz, Jean-Louis Mandel, Petra Kioschis, Johannes F. Coy, Sabine M. Klauck, Annemarie Poustka, Niklas Dahl, A gene mutated in X–linked myotubular myopathy defines a new putative tyrosine phosphatase family conserved in yeast Nature Genetics. ,vol. 13, pp. 175- 182 ,(1996) , 10.1038/NG0696-175
Georges Imbert, Frédéric Saudou, Gaël Yvert, Didier Devys, Yvon Trottier, Jean-Marie Garnier, Chantal Weber, Jean-Louis Mandel, Gëraldine Cancel, Nacer Abbas, Alexandra Dürr, Olivier Didierjean, Giovanni Stevanin, Yves Agid, Alexis Brice, Cloning of the gene for spinocerebellar ataxia 2 reveals a locus with high sensitivity to expanded CAG/glutamine repeats Nature Genetics. ,vol. 14, pp. 285- 291 ,(1996) , 10.1038/NG1196-285
Darrell O. Ricke, Qiang Liu, Bobbie Gostout, Steve S. Sommer, Nonrandom patterns of simple and cryptic triplet repeats in coding and noncoding sequences Genomics. ,vol. 26, pp. 510- 520 ,(1995) , 10.1016/0888-7543(95)80169-M
James P. Carney, Christopher McKnight, Steffany Vanepps, Mark R. Kelley, Random rapid amplification of cDNA ends (RRACE) allows for cloning of multiple novel human cDNA fragments containing (CAG)n repeats Gene. ,vol. 155, pp. 289- 292 ,(1995) , 10.1016/0378-1119(94)00758-K
R. Koide, T. Ikeuchi, O. Onodera, H. Tanaka, S. Igarashi, K. Endo, H. Takahashi, R. Kondo, A. Ishikawa, T. Hayashi, M. Saito, A. Tomoda, T. Miike, H. Naito, F. Ikuta, S. Tsuji, Unstable expansion of CAG repeat in hereditary dentatorubral–pallidoluysian atrophy (DRPLA) Nature Genetics. ,vol. 6, pp. 9- 13 ,(1994) , 10.1038/NG0194-9