Trypsin Digestion in Immunoperoxidase Staining
作者： N W Hautzer , J F Wittkuhn , W T Mccaughey
DOI: 10.1177/28.1.6153193
关键词: Trypsin Digestion 、 Paraffin section 、 Immunoperoxidase Staining 、 Biochemistry 、 Staining 、 Trypsin 、 Molecular biology 、 Specific staining 、 Biology
摘要: The effect of trypsin digestion on the immunoperoxidase-PAP staining paraffin sections from normal and abnormal tissue was studied. Trypsin treatment reduced background considerably, but enhanced specific staining. Results were, therefore, much easier to read. Possible reasons for this result are discussed.
sagepub.com
sci-hub.se 参考文章(10)
Minassian H, Huang Sn, More Jd, Application of immunofluorescent staining on paraffin sections improved by trypsin digestion. Laboratory Investigation. ,vol. 35, pp. 383- ,(1976)
CliveR Taylor, THE NATURE OF REED-STERNBERG CELLS AND OTHER MALIGNANT " RETICULUM " CELLS The Lancet. ,vol. 304, pp. 802- 807 ,(1974) , 10.1016/S0140-6736(74)91071-X
M Reading, A digestion technique for the reduction of background staining in the immunoperoxidase method. Journal of Clinical Pathology. ,vol. 30, pp. 88- 90 ,(1977) , 10.1136/JCP.30.1.88
H. Denk, T. Radaszkiewicz, E. Weirich, Pronase pretreatment of tissue sections enhances sensitivity of the unlabelled antibody-enzyme (PAP) technique Journal of Immunological Methods. ,vol. 15, pp. 163- 167 ,(1977) , 10.1016/0022-1759(77)90027-8
R. C. Curran, J. Gregory, The unmasking of antigens in paraffin sections of tissue by trypsin. Cellular and Molecular Life Sciences. ,vol. 33, pp. 1400- 1401 ,(1977) , 10.1007/BF01920206
R C Curran, J Gregory, Demonstration of immunoglobulin in cryostat and paraffin sections of human tonsil by immunofluorescence and immunoperoxidase techniques. Effects of processing on immunohistochemical performance of tissues and on the use of proteolytic enzymes to unmask antigens in sections Journal of Clinical Pathology. ,vol. 31, pp. 974- 983 ,(1978) , 10.1136/JCP.31.10.974
J W Bigbee, J C Kosek, L F Eng, Effects of primary antiserum dilution on staining of "antigenrich" tissues with the peroxidase antiperoxidase technique. Journal of Histochemistry and Cytochemistry. ,vol. 25, pp. 443- 447 ,(1977) , 10.1177/25.6.69655
Clive R. Taylor, Raymond Russell, Stebbins Chandor, An immunohistologic study of multiple myeloma and related conditions, using an immunoperoxidase method. American Journal of Clinical Pathology. ,vol. 70, pp. 612- 622 ,(1978) , 10.1093/AJCP/70.4.612
Roger Warnke, Marilyn Pederson, Christopher Williams, Ronald Levy, A Study of Lymphoproliferative Diseases Comparing Immunofluorescence with Immunohistochemistry American Journal of Clinical Pathology. ,vol. 70, pp. 867- 875 ,(1978) , 10.1093/AJCP/70.6.867
Huang Sn, Immunohistochemical demonstration of hepatitis B core and surface antigens in paraffin sections. Laboratory Investigation. ,vol. 33, pp. 88- 95 ,(1975)