Differentially expressed gene transcripts in roots of resistant and susceptible chickpea plant (Cicer arietinum L.) upon Fusarium oxysporum infection
作者： Suhas B. Nimbalkar , Abhay M. Harsulkar , Ashok P. Giri , Mohini N. Sainani , Vincent Franceschi
DOI: 10.1016/J.PMPP.2006.10.003
关键词: Complementary DNA 、 Reverse northern blot 、 WRKY protein domain 、 Gene 、 Fusarium oxysporum 、 Fusarium 、 Biology 、 Genetics 、 Botany 、 RAPD 、 Gene expression
摘要: Abstract Differentially expressed genes in chickpea, ( Cicer arietinum L.) during root infection by Fusarium oxysporum f sp. ciceri Race1, were identified using cDNA-RAPD and cDNA-AFLP approaches. The former employed decamer primers on cDNA template revealed nine differentially transcripts the resistant-infected chickpea variety. Among 2000 transcript-derived fragments (TDFs) screened cDNA-AFLP, 273 roots infection. Only 13.65% of TDFs regulated pathogen challenge, while other 86% non-differentially process roots. Nineteen TDFs, which variety cloned sequenced. Two these similar to transcription factors like WRKY proteins 14-3-3 proteins, three represented NBS-LRR-type gene sequences. had sequence identity known have function defense. RAPD TDF CaFRi60 showed gamma-glutamyl-cysteine synthetase. examined 19 confirmed Reverse Northern blot be expressed. data confirms effectiveness technique detecting pathogenesis.
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