The Neonatal Ventromedial Hypothalamus Transcriptome Reveals Novel Markers with Spatially Distinct Patterning

摘要: The ventromedial hypothalamus (VMH) is a distinct morphological nucleus involved in feeding, fear, thermoregulation, and sexual activity. It essentially unknown how VMH circuits underlying these innate responses develop, part because the remains poorly defined at cellular molecular level. Specifically, there paucity of cell-type-specific genetic markers with which to identify neuronal subgroups manipulate development signaling vivo . Using gene profiling, we now ∼200 genes highly enriched neonatal (postnatal day 0) mouse tissue. Analyses by real or virtual (Allen Brain Atlas; http://www.brain-map.org) experiments revealed regional patterning within newly formed VMH. Top include transcriptional regulators such as Vgll2, SF-1, Sox14, Satb2, Fezf1, Dax1, Nkx2-2, COUP-TFII, but interestingly, highest expressed transcript, coregulator completely absent older animals. Collective results from zebrafish knockdown studies suggest that subset will be important for hypothalamic downstream critical factor normal differentiation. We show least one marker, AT-rich binding protein was responsive loss leptin ( Lep ob/ob ) postnatal 0 not adult, suggesting some programs might influenced fetal early environments. Our study describing this comprehensive “VMH transcriptome” provides novel toolkit probe further basis neuroendocrine behavioral responses.