Adult equine bone-marrow stromal cells produce a cartilage-like ECM superior to animal-matched adult chondrocytes

摘要: Our objective was to evaluate the age-dependent mechanical phenotype of bone marrow stromal cell(BMSC-) and chondrocyte-produced cartilage-like neotissue elucidate matrix-associated mechanisms which generate this phenotype. Cells from both immature (2-4 month-old foals) skeletally-mature (2-5 year-old adults) mixed-breed horses were isolated animal-matched cartilage tissue, encapsulated in self-assembling-peptide hydrogels, cultured with without TGF-β1 supplementation. BMSCs chondrocytes donor ages high viability. produced neo-tissue higher stiffness than that by either young or adult chondrocytes. Young, but not adult, proliferated response while age groups TGF-β1. Young stimulated accumulated ECM 10-fold sulfated-glycosaminoglycan content 2-3-fold age. The opposite trend observed for hydroxyproline content, accumulating more chondrocytes, independent Size-exclusion chromatography extracted proteoglycans showed an aggrecan-like peak predominant sulfated proteoglycan all cell types. Direct measurement aggrecan core protein length chondroitin sulfate chain single molecule atomic force microscopy imaging revealed that, age, longer chains, fewer short molecules suggesting BMSC-produced has a © 2009 Elsevier B.V. All rights reserved. Corresponding author: Alan J. Grodzinsky Department Biological Engineering MIT Center Biomedical Massachusetts Institute Technology 77 Ave, Rm. NE47-377 Cambridge, MA 02139 phone: 617-253-4969 fax: 617-258-5239 alg@mit.edu. Publisher's Disclaimer: This is PDF file unedited manuscript been accepted publication. As service our customers we are providing early version manuscript. will undergo copyediting, typesetting, review resulting proof before it published its final citable form. Please note during production process errors may be discovered could affect legal disclaimers apply journal pertain. NIH Public Access Author Manuscript Matrix Biol. manuscript; available PMC 2011 June 1. Published edited form as: 2010 ; 29(5): 427–438. doi:10.1016/j.matbio.2010.02.003. N IH PA Athor M anscript characteristic tissue aggrecan. Aggrecan ultrastructure, composition, cellular proliferation combine suggest mechanism produce superior