Induction of IL-10-producing CD4+CD25+ T cells in animal model of collagen-induced arthritis by oral administration of type II collagen

摘要: Induction of oral tolerance has long been considered a promising approach to the treatment chronic autoimmune diseases, including rheumatoid arthritis (RA). Oral administration type II collagen (CII) proven improve signs and symptoms in RA patients without troublesome toxicity. To investigate mechanism immune suppression mediated by orally administered antigen, we examined changes serum IgG subtypes T-cell proliferative responses CII, generation IL-10-producing CD4+CD25+ subsets an animal model collagen-induced (CIA). We found that joint inflammation CIA mice peaked at 5 weeks after primary immunization with which was significantly less tolerized repeated feeding CII before induction. Mice had fed also exhibited increased IgG1 decreased IgG2a as compared nontolerized animals. The response suppressed lymph nodes also. Production IL-10 transforming growth factor-β from mononuclear lymphocytes animals, CD4+ T cells isolated did not respond induction IFN-γ when stimulated vitro CII. observed greater among CII-stimulated splenic mice. These data suggest these encounter antigen affected joints they become activated exert anti-inflammatory effect.