Connexin 46 and connexin 50 in selenite cataract.
作者： C.R. Fleschner
DOI: 10.1159/000088527
关键词: Biochemistry 、 Phosphorylation 、 Gap junction 、 Cortex (botany) 、 Chemistry 、 Blot 、 Nucleus 、 Proteolysis 、 Molecular biology 、 Connexin 、 Selenium
摘要: The purpose of this work was to determine if the lens gap junction proteins connexin 46 (Cx46) and 50 (Cx50) were altered with development selenite-induced cataract. Cataracts induced in young Sprague-Dawley rats a single subcutaneous injection sodium selenite; age-matched uninjected served as controls. Membrane fractions isolated from homogenates cortex nucleus normal cataractous lenses by differential discontinuous sucrose gradient centrifugation. Aliquots urea-insoluble protein membrane analyzed quantitative densitometry Western blots probed antibodies Cx46 Cx50. A significant decrease more slowly migrating Cx46-reactive band, which represents phosphorylated Cx46, found major fraction lenses. There no difference amounts either or Cx50 associated selenite cataract any examined. These results suggest that alteration function (as evidenced change phosphorylation Cx46) may be cataract, but neither nor is subject well-characterized proteolysis model.
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sci-hub.se 参考文章(22)
L L David, T R Shearer, Calcium-activated proteolysis in the lens nucleus during selenite cataractogenesis. Investigative Ophthalmology & Visual Science. ,vol. 25, pp. 1275- 1283 ,(1984)
Takemoto Dj, Takemoto Lj, Saleh Sm, Zoukhri D, PKC-gamma phosphorylation of connexin 46 in the lens cortex. Molecular Vision. ,vol. 7, pp. 240- ,(2001)
Jean X Jiang, David L Paul, Daniel A Goodenough, None, Posttranslational phosphorylation of lens fiber connexin46: A slow occurrence Investigative Ophthalmology & Visual Science. ,vol. 34, pp. 3558- 3565 ,(1993)
Thomas W White, Daniel A Goodenough, David L Paul, None, Targeted Ablation of Connexin50 in Mice Results in Microphthalmia and Zonular Pulverulent Cataracts Journal of Cell Biology. ,vol. 143, pp. 815- 825 ,(1998) , 10.1083/JCB.143.3.815
T W White, R Bruzzone, D A Goodenough, D L Paul, Mouse Cx50, a functional member of the connexin family of gap junction proteins, is the lens fiber protein MP70. Molecular Biology of the Cell. ,vol. 3, pp. 711- 720 ,(1992) , 10.1091/MBC.3.7.711
Dingbo Lin, Daniel L. Boyle, Dolores J. Takemoto, IGF-I-induced phosphorylation of connexin 43 by PKCγ: Regulation of gap junctions in rabbit lens epithelial cells Investigative Ophthalmology & Visual Science. ,vol. 44, pp. 1160- 1168 ,(2003) , 10.1167/IOVS.02-0737
Kenneth Hightower, Janet McCready, Selenite-lnduced Damage to Lens Membranes Experimental Eye Research. ,vol. 58, pp. 225- 229 ,(1994) , 10.1006/EXER.1994.1011
Marjorie B. Lees, Sally Paxman, Modification of the Lowry procedure for the analysis of proteolipid protein. Analytical Biochemistry. ,vol. 47, pp. 184- 192 ,(1972) , 10.1016/0003-2697(72)90291-6
Donna Mackay, Alexander Ionides, Zoha Kibar, Guy Rouleau, Vanita Berry, Anthony Moore, Alan Shiels, Shomi Bhattacharya, Connexin46 Mutations in Autosomal Dominant Congenital Cataract The American Journal of Human Genetics. ,vol. 64, pp. 1357- 1364 ,(1999) , 10.1086/302383
Xiaohua Gong, En Li, George Klier, Qingling Huang, Ying Wu, Hong Lei, Nalin M Kumar, Joseph Horwitz, Norton B Gilula, Disruption of α3 connexin gene leads to proteolysis and cataractogenesis in mice Cell. ,vol. 91, pp. 833- 843 ,(1997) , 10.1016/S0092-8674(00)80471-7