Regulatory effect of small interfering RNA targeting multidrug resistant protein 1 on chemosensitivity of human multiforme glioblastoma cell line BT325

摘要: BACKGROUND & OBJECTIVE Multidrug resistance is a major reason of failure chemotherapy for glioma. Overexpression P-glycoprotein (P-gp), encoded by multidrug resistant protein 1 (MDR1) gene, one the key factors. This study was to explore regulatory effect small interfering RNA (siRNA) targeting MDR1 on chemosensitivity human multiforme glioblastoma cell line BT325. METHODS siRNAs containing sequences 3,051-3,069 (MDR1 A group), 502-520 B and 1,534-1,552 C group) were designed, transfected into BT325 cells. Positive clones screened with puromycin. The expression measured reverse transcription-polymerase chain reaction (RT-PCR); P-gp detected immunohistochemistry flow cytometry (FCM). Drug sensitivity assay performed in RESULTS cells proliferated exponentially after siRNA transfection. After transfection siRNAs, mRNA significantly lower A, B, groups than control group (0.18+/-0.05, 0.30+/-0.09, 0.36+/-0.13 vs. 0.76+/-0.06, P<0.001); positive rate decreased from 85.73% 1.44%; 50% inhibitory concentrations (IC(50)) doxorubicin vincristine markedly; G0/G1 phase proportions increased 13.55%, 14.35%, 1.46% control, respectively (P<0.05). CONCLUSION may modulate through down-regulating enhancing glioma, inducing apoptosis.