Use of the DiaMed Impact R to test platelet function in stored platelet concentrates.

作者: A. Morrison , V. S. Hornsey , C. V. Prowse , I. R. MacGregor

DOI: 10.1111/J.1423-0410.2007.00938.X

关键词: PlateletImmunologyPlatelet adhesionPhysiological flowPlatelet concentrateChromatographyPlatelet storageWhole bloodAverage sizeAdhesionChemistry

摘要: BACKGROUND AND OBJECTIVES The DiaMed Impact R tests platelet function under close to physiological flow conditions using cone and plate technology. An image analyser quantifies the adhered platelets results are expressed as percentage of well surface covered by aggregates (SC %) an index adhesion average size (AS microm(2)) aggregation. machine is designed use whole blood aim this study was determine if it could be used assess in concentrates (PC). MATERIAL METHODS Platelet were mixed with various ratios platelet-poor plasma (PPP) or ABO-compatible routine leucoreduced red cells saline-adenine-glucose-mannitol. effects counts, haematocrit, shear rate time activation upon SC AS evaluated identify optimized assay conditions. Routine PCs then tested on Days 2, 5 7. Samples also stored at 4 37 degrees C for 1 2 h before see altered. RESULTS concentrate PPP resulted no detectable adhesion. However, addition PC measurable Optimal identified 1800 per second, 4-min activation, count between 250 400 x 10(6) ml, haematocrit 30 40%. When these we observed median values 8.2% 32.7 microm(2) days, which reduced 6.9% 25.0 microm(2), respectively, after 5-day storage 6.8% 21.0 7 days. CONCLUSION We able reconstitute adding allow aggregation functions R. shown decrease during but improved a 1-h treatment C.

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