Ex vivo evaluation of erythrocytosis‐enhanced platelet thrombus formation using the cone and plate(let) analyzer: effect of platelet antagonists

摘要: Red blood cells (RBC) contribute significantly to haemostasis and thrombosis under oscillatory flow conditions, erythrocytosis has been associated with increased thrombotic risk. To measure the dynamic influences of RBC on platelets, we used a recently described cone plate(let) analyzer (CPA), evaluating effect haematocrit (Hct) platelet function in whole arterial conditions (1800/s, 2 min, 25 degrees C). Anticoagulated blood, reconstituted varying haematocrits autologous RBC, demonstrated significant increase adherent aggregate formation at Hct levels >45%. This was not affected by pretreatment 0.05 mmol/l aspirin, but prevented antagonists P2Y1, P2Y12, or P2X1, ADP ATP receptors, converting exogenous creatine phosphate/creatine phosphokinase. As negligible granule secretion measured during CPA analysis, metabolic inhibition sodium azide glutaraldehyde fixation inhibited erythrocytosis-enhanced increases size, adenine nucleotides contributing shear-induced appear be derived from erythrocytes. These findings support use for ex vivo evaluation contribution its physiological shear conditions.