Potentiation of platelet activation through the stimulation of P2X1 receptors.

摘要: Summary.  The platelet P2X1 purinergic receptor is a ligand-gated ion channel that responds to ATP. precise role of in function unknown, though stimulation with the agonist α,β-Me-ATP known result shape change through elevation calcium levels. aim present study was examine further effects on activation. Stimulation resulted and small aggregate formation heparin-anticoagulated preparations. Given ability heparin potentiate activation, subsequent experiments were performed hirudin. In these preparations, response alone less than observed heparin; however, significantly potentiated when added conjunction other weak agonists [epinephrine or thrombopoietin (TPO)]. Platelet confirmed by single loss (microaggregate formation), microscopy, light transmittance studies. Further, antagonist MRS-2159 inhibited aggregation responses induced α,β-Me-ATP. Overall, this demonstrates can induce/potentiate activation combination agonists. These results are first demonstration mediated direct stimulation, supporting for regulating