Glucose-Sensing Electrode Based on Glucose Oxidase-Attached Polyion Complex Membrane Containing Peroxidaseand Ferrocene
作者： Soichi Yabuki , Fumio Mizutani , Yoshiki Hirata
DOI: 10.1002/1521-4109(200104)13:5<380::AID-ELAN380>3.0.CO;2-6
关键词: Membrane 、 Glucose Measurement 、 Electrode 、 Enzyme electrode 、 Biosensor 、 Chromatography 、 Glucose oxidase 、 Glutaraldehyde 、 Nuclear chemistry 、 Ferrocene 、 Chemistry 、 Analytical chemistry 、 Electrochemistry
摘要: A glucose oxidase (GOD)-attached polyion complex membrane containing peroxidase (POD) and ferrocene was prepared by two steps on a glassy carbon electrode. First, POD prepared: poly-L-lysine solution, dispersion poly(styrenesulfonate) solution were successively dropped an electrode, the electrode allowed to dry. Then GOD attached use of glutaraldehyde: glutaraldehyde solutions membrane, dried. Current response for enzyme measured at potential −0.2 V (vs. Ag/AgCl). The 100 % time ca. 20 s. current proportional concentration up 0.1 mM. lower detection limit 1 µM (signal-to-noise ratio, S/N=5). used measurements in beverages. results obtained agreed well with those Boehringer F-kit method. would be useful as biosensor
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