Direct analysis of solute self-association by sedimentation equilibrium
作者： Peter R. Wills , Michael P. Jacobsen , Donald J. Winzor
DOI: 10.1002/(SICI)1097-0282(199601)38:1<119::AID-BIP10>3.0.CO;2-C
关键词: Chemistry 、 Direct analysis 、 Activity coefficient 、 Allowance (engineering) 、 Solution composition 、 Variable (mathematics) 、 Self association 、 Sedimentation equilibrium 、 Thermodynamics
摘要: An improved procedure is described for the characterization of solute self-association by sedimentation equilibrium, Whereas previous statistical-mechanical approaches to allowance effects thermodynamic nonideality have entailed tedious iteration because their specification activity coefficients in terms equilibrium concentrations all species, such reliance upon knowledge solution composition avoided adaptation an alternative formulation [T. L. Hill and Y. D. Chen (1973) Biopolymers, Vol. 12, pp. 1285-1312 ] which expressed total concentration. The development analysis a relationship with concentration as experimental variable allows this attribute Adams-Fujita approach be retained without sacrifice rigor. Its use illustrated application Rayleigh interferometric records distributions reflecting α-chymotrypsin dimerization lysozyme self-association.
uq.edu.au参考文章(31)
Donald J. Winzor, Peter R. Wills, The Omega Analysis and the Characterization of Solute Self-Association by Sedimentation Equilibrium Modern Analytical Ultracentrifugation. pp. 66- 80 ,(1994) , 10.1007/978-1-4684-6828-1_4
Arthur Jennison Rowe, S. E. Harding, J. C. Horton, Analytical ultracentrifugation in biochemistry and polymer science Royal Society of Chemistry. ,(1992)
Terrell L. Hill, Thermodynamics for Chemists and Biologists ,(1968)
Edward F. Casassa, Thermodynamic Analysis of Multicomponent Solutions Advances in Protein Chemistry. ,vol. 19, pp. 287- 395 ,(1964) , 10.1016/S0065-3233(08)60191-6
George D. J. Phillies, Effects of intermacromolecular interactions on diffusion. I. Two‐component solutions The Journal of Chemical Physics. ,vol. 60, pp. 976- 982 ,(1974) , 10.1063/1.1681177
Terrell L. Hill, J. Gillis, An Introduction to Statistical Thermodynamics ,(1960)
Serge N. Timasheff, Kirk C. Aune, Lowell C. Goldsmith, DIMERIZATION OF $alpha$-CHYMOTRYPSIN. II. IONIC STRENGTH AND TEMPERATURE DEPENDENCE. Biochemistry. ,vol. 10, pp. 1617- 1622 ,(1971) , 10.1021/BI00785A018
David A. Yphantis, Equilibrium Ultracentrifugation of Dilute Solutions Biochemistry. ,vol. 3, pp. 297- 317 ,(1964) , 10.1021/BI00891A003
Thomas A. Horbett, Daivd C. Tller, Low pH dimerization behavior of active-site derivatives of bovine -chymotrypsin. Biochemistry. ,vol. 12, pp. 1349- 1358 ,(1973) , 10.1021/BI00731A015
P.R. Willis, L.W. Nichol, R.J. Siezen, The indefinite self-association of lysozyme: consideration of composition-dependent activity coefficients Biophysical Chemistry. ,vol. 11, pp. 71- 82 ,(1980) , 10.1016/0301-4622(80)85009-5