Independent expression of the adrenergic phenotype by neural crest cells in vitro

摘要: Abstract Neural crest cells obtained from Japanese quail and grown in vitro without other embryonic tissues differentiate into adrenergic cells. These show intense catecholamine-specific histochemical fluorescence, some have long, varicose neuronal processes. Ultrastructural examination shows two populations of cells, one with small (about 90 nm) dense-core vesicles resembling principal sympathetic neurons the larger 150 granules chromaffin or intensely fluorescent Neuronal characteristics are also present. results compatible hypothesis that a population determined along lines exists neural prior to migration.