Cisplatin, doxorubicin and paclitaxel induce mdr1 gene transcription in ovarian cancer cell lines.

摘要: The clinical observation of the multidrug resistance (MDR) phenotype is often associated with overexpression mdr1 gene, in particular respect to ovarian cancer. However, until now mdr1-inducing potential commonly used antineoplastics has been only incompletely explored. We performed short-term cultures six cancer cell lines (MZOV4, EFO27, SKOV3, OAW42, OTN14, MZOV20) exposed either blank medium or cisplatin, doxorubicin paclitaxel at concentrations related clinically achievable plasma peak concentration. A highly specific quantitative real-time RT-PCR was detect Mdr1 transcripts. mRNA contents were calibrated relation coamplified GAPDH mRNA. detectable each line. In 13 out 18 assays (72%) anticancer drug being tested induced transcription. No decrease concentration observed. Our data suggest that induction by one reasons for failure therapy but may vary individually.