A Prominin-1-Rich Pediatric Glioblastoma: Biologic Behavior Is Determined by Oxygen Tension-Modulated CD133 Expression but Not Accompanied by Underlying Molecular Profiles

摘要: Few studies on the biologic and molecular properties of pediatric glioblastoma have been performed. Until now, differential genomic analysis CD133+ve CD133-ve fractions has not described in glioma. We hypothesize only that presence CD133 could be source tumor resistance but also maintenance this molecule by hypoxia dictates cellular behavior. From a series human biopsies investigated, one, IN699 (from glioblastoma), generated greater than 4% total cell volume as cells. Using glioblastoma, containing unprecedented high levels putative brain stem marker CD133, study model, we report characteristics parent culture populations derived therefrom under atmospheric hypoxic conditions. Immunocytochemistry flow cytometry were performed with antigenic markers known to characterize neural cells associated glioma Behavioral was carried out using proliferation, adhesion, migration, invasion assays. Cell cycle array comparative hybridization used assess copy number profiles for parental fractions, respectively. With regard inversely proportional, significant increase invasive propensity within (P < .005) proliferation .005). Our observations indicate identical imbalances between fractions. Furthermore, our research documents direct link decreasing oxygen tension expression.