Combinatorial approaches to evaluate nanodiamond uptake and induced cellular fate

摘要: Nanodiamonds (NDs) are an emerging class of engineered nanomaterials that hold great promise for the next generation bionanotechnological products to be used drug and gene delivery, or bio-imaging biosensing. Previous studies have shown upon their cellular uptake, NDs exhibit high biocompatibility in various vitro vivo set-ups. Herein we hypothesized increased is a result minimum membrane perturbations reduced ability induce disruption damage during translocation. Using multi-scale combinatorial approaches simulate ND-membrane interactions, correlated real-time uptake kinetics with ND-induced fluctuations derive energy requirements occur. Our discrete analyses showed majority internalization occurs within 2 h exposure, however, no effects on viability, proliferation behavior. Furthermore, our simulation using coarse-grained models identified key changes profile, deformation recovery time, all functions average ND ND-based agglomerate size. Understanding mechanisms responsible ND-cell interactions could possibly advance implementation biomedical applications.