Co-delivery of paclitaxel and cetuximab by nanodiamond enhances mitotic catastrophe and tumor inhibition.
作者： Yu-Wei Lin , Emmanuel Naveen Raj , Wei-Siang Liao , Johnson Lin , Kuang-Kai Liu
DOI: 10.1038/S41598-017-09983-8
关键词:
摘要: The poor intracellular uptake and non-specific binding of anticancer drugs into cancer cells are the bottlenecks in therapy. Nanocarrier platforms provide opportunities to improve drug efficacy. Here we show a carbon-based nanomaterial nanodiamond (ND) that carried paclitaxel (PTX), microtubule inhibitor, cetuximab (Cet), specific monoclonal antibody against epidermal growth factor receptor (EGFR), inducing mitotic catastrophe tumor inhibition human colorectal (CRC). ND-PTX blocked progression, chromosomal separation, induced apoptosis CRC cells; however, NDs did not induce these effects. Conjugation with Cet (ND-PTX-Cet) was specifically EGFR-positive enhanced induction. Besides, ND-PTX-Cet markedly decreased size xenograft EGFR-expressed tumors nude mice. Moreover, marker protein phospho-histone 3 (Ser10) apoptotic active-caspase for apoptosis. Taken together, this study demonstrated co-delivery PTX by ND effects vitro vivo, which may be applied
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