Cymbidium mosaic potexvirus isolate-dependent host movement systems reveal two movement control determinants and the coat protein is the dominant

摘要: Little is known about how plant viruses of a single species exhibit different movement behavior in host species. Two Cymbidium mosaic potexvirus (CymMV) isolates, M1 and M2, were studied. Both can infect Phalaenopsis orchids, but only systemically Nicotiana benthamiana plants. Protoplast inoculation whole-mount situ hybridization revealed that both isolates replicate N. benthamiana; however, M2 was restricted to the initially infected cells. Genome shuffling between two control modes are involved CymMV dependent movement. The coat protein (CP) plays dominant role controlling cells, because all chimeric containing CP Without CP, one virus combination triple gene block proteins (TGBps), 5' RNA (1-4333), effectively moved Further complementation analysis TGBp1 TGBp3 co-required complement benthamiana. amino acids within which required or important for plants mapped. map predicted binding domain. RNA-protein assays has higher affinity than does CP. Yeast two-hybrid detect possible interactions TGBps self-interactions observed.