Titanium with nanotopography induces osteoblast differentiation through regulation of integrin αV.

摘要: Topographical modifications of titanium (Ti) at the nanoscale level generate surfaces that regulate several signaling pathways and cellular functions, which may affect process osseointegration. Here, we investigated participation integrin αV in osteogenic capacity Ti with nanotopography. Machined discs (untreated) were submitted to treatment H2 SO4 /H2 O2 produce nanotopography (nanostructured). First, greater increased osteoblast differentiation mesenchymal stem cells compared untreated topography was shown. Also, nanostructured surface (regulation ≥ 1.9-fold) gene expression 6 integrins from a custom array plate utilized evaluate 84 genes correlated cell adhesion pathway, including αV, is involved differentiation. By silencing MC3T3-E1 cultured on nanotopography, impairment induced by this observed. In conclusion, it shown regulates components pathway its higher potential is, least part, due ability upregulate αV. Together previous data showed α1, β1, β3 osseoinduction activity, have uncovered pivotal role family membrane receptors surface.