The Wnt/β-catenin signaling pathway is regulated by titanium with nanotopography to induce osteoblast differentiation
作者： Rodrigo PF Abuna , Fabiola S Oliveira , Helena B Lopes , Gileade P Freitas , Roger R Fernandes
DOI: 10.1016/J.COLSURFB.2019.110513
关键词:
摘要: Wnt/β-catenin signal transduction is involved in the homeostatic control of bone mass. It well established that a titanium surface with nanotopography (Ti-Nano) favors osteoblast differentiation by modulating different signaling pathways. However, few studies have investigated participation pathway osteogenic effect nanoscale topographies. In this study, we aimed to determine whether elevated potential Ti-Nano. MC3T3-E1 cells were cultured on Ti-Nano and machined Ti (Ti-Control) for evaluation expression pathway-related genes. Based results real-time PCR, Wnt receptor Fzd4 was selected silenced CRISPRi. The resulting both surfaces, several events evaluated. revealed gene silencing, corresponding negative modulation Wnt/β-catenin, inhibits phenotype. worthy note inhibitory more pronounced grown compared those Ti-Control. By disrupting expression, shown due activation pathway, which reveals new mechanism explain induced nanotopography. Such an understanding intracellular machinery guiding fate may contribute development smart biomaterials modulate process implant osseointegration.
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