Proteoglycans of rabbit corneal stroma. Isolation and partial characterization.
作者： J D Gregory , L Cöster , S P Damle
DOI: 10.1016/S0021-9258(18)34524-1
关键词: Iduronic acid 、 Biochemistry 、 Glucosamine 、 Glycosaminoglycan 、 Amino acid 、 Chemistry 、 Gel permeation chromatography 、 Uronic acid 、 Papain 、 Sialic acid
摘要: Proteoglycans extracted from rabbit corneal stroma can be separated by ion exchange and gel chromatography into two proteokeratan sulfates (PKS-I, PKS-II) proteodermatan (PDS-I, PDS-II). PKS-I (21% of the total glycosaminoglycans) contains 48% protein, PKS-II (43% total) has 57% protein. In both, only hexosamine is glucosamine, 7% which found in oligosaccharides. There much more sialic acid oligosaccharides than PKS-I, amino compositions proteoglycans differ significantly. The keratan isolated them papain digestion are different size, but both digested endo-beta-galactosidase. PDS-I (30% 32% 35% its uronic iduronic acid. PDS-II (6% 42% as iduronic. general, four resembled those human bovine being smaller protein-rich cartilage.
sci-hub.st 参考文章(46)
Y Oike, K Kimata, T Shinomura, S Suzuki, Proteinase activity in chondroitin lyase (chondroitinase) and endo-beta-D-galactosidase (keratanase) preparations and a method to abolish their proteolytic effect on proteoglycan. Biochemical Journal. ,vol. 191, pp. 203- 207 ,(1980) , 10.1042/BJ1910203
Zacharias Dische, Gertrud Cremer-Bartels, Gordon I. Kaye, Nancy W. Kaye, Erhardt Buddeke, Metabolic Heterogeneity of Keratan Sulfate in Bovine Cornea and its Role in the Maintenance of Corneal Collagen Structure Springer Netherlands. pp. 253- 262 ,(1979) , 10.1007/978-94-009-9609-0_29
I Axelsson, D Heinegård, Fractionation of proteoglycans from bovine corneal stroma. Biochemical Journal. ,vol. 145, pp. 491- 500 ,(1975) , 10.1042/BJ1450491
Proteoglycans from chick limb bud chondrocyte cultures. Keratan sulfate and oligosaccharides which contain mannose and sialic acid Journal of Biological Chemistry. ,vol. 255, pp. 6077- 6083 ,(1980) , 10.1016/S0021-9258(18)43703-9
P J Roughley, D McNicol, Extraction and characterization of proteoglycan from human meniscus. Biochemical Journal. ,vol. 185, pp. 705- 713 ,(1980) , 10.1042/BJ1850705
T. R. Oegema, R. Eisenstein, Vincent C Hascall, Characterization of bovine aorta proteoglycan extracted with guanidine hydrochloride in the presence of protease inhibitors. Journal of Biological Chemistry. ,vol. 254, pp. 1312- 1318 ,(1979) , 10.1016/S0021-9258(17)34204-7
Karl Meyer, Alfred Linker, Eugene A. Davidson, Bernard Weissmann, The mucopolysaccharides of bovine cornea. Journal of Biological Chemistry. ,vol. 205, pp. 611- 616 ,(1953) , 10.1016/S0021-9258(18)49203-4
S.P. Damle, F.J. Kieras, W.K. Tzeng, J.D. Gregory, Isolation and characterization of proteochondroitin sulfate from pig skin. Journal of Biological Chemistry. ,vol. 254, pp. 1614- 1620 ,(1979) , 10.1016/S0021-9258(17)37816-X
Torvard C. Laurent, Arvid Anseth, Studies on corneal polysaccharides Experimental Eye Research. ,vol. 1, pp. 99- 105 ,(1961) , 10.1016/S0014-4835(61)80014-6
Arvid Anseth, Lars-ke Fransson, Studies on corneal polysaccharides: VI. Isolation of dermatan sulfate from corneal scar tissue* Experimental Eye Research. ,vol. 8, pp. 302- 309 ,(1969) , 10.1016/S0014-4835(69)80043-6