A comparative biochemical and ultrastructural study of proteoglycan-collagen interactions in corneal stroma. Functional and metabolic implications.

摘要: 1. Corneas of mouse, rat, guinea pig, rabbit, sheep, cat, dog, pig and cow were quantitatively analysed for water, hydroxyproline, nucleic acid, total sulphated polyanion, chondroitin sulphate/dermatan sulphate keratan sulphate, several samples or pools tissue from each species being used. Ferret cornea was similarly water hydroxyproline on one pool eight corneas. Pooled frog (38) ferret (eight) corneas a single sample human qualitatively examined by electrophoresis cellulose acetate membranes. Nine (mouse, frog, cow) light microscopy six rabbit electron microscopy, with the use Alcian Blue Cupromeronic in critical-electrolyte-concentration (CEC) methods to stain proteoglycans. 2. Water (% wet weight), (mg/g dry wt.) hydroxyproline) contents approximately constant across species, except mouse. 3. Keratan increased corneal thickness, whereas dermatan decreased. The oversulphated domain absent mouse corneas, increasing as percentage thickness. Sulphation essentially complete (i.e. group per disaccharide unit). 4. Chondroitin proteoglycans present at d bands collagen fibrils all examined, orthogonally arrayed, high frequency, occasionally e bands. c but far higher frequency thicker where high. 5. CEC staining showed much sulphation thick e.g. that cow, than thin keeping biochemical analyses. 6. It is suggested constancy interfibrillar volumes regulated via swelling osmotic pressure polyanions, adjustment extent two independent proteoglycan populations, achieve an 'average sulphation' polyanion similar fully sulphate. 7. balance synthesis kinds may be determined O2 supply avascular cornea. also determine conversion into