Vacuolar-type H+-ATPase E subunit is required for embryogenesis and yolk transfer in Caenorhabditis elegans

摘要: Abstract Vacuolar H+-ATPases (V-ATPases) are ATP-dependent proton pumps localized at membranes of intracellular acidic organelles and plasma various cell types. By virtue its regulation in acidification, V-ATPase is required for many processes such as receptor-mediated endocytosis protein sorting. Here we report the molecular characterization E subunit Caenorhabditis elegans. This one most well conserved subunits sharing approximately 57% identity with human homologue, ATP6E. Green fluorescent (GFP) whole-mount immunostaining analyses showed that (vha-8) abundantly expressed H-shaped excretory cell, consistent expression patterns observed other subunits. Double-stranded RNAs (or RNAi) targeted to vha-8 resulted embryonic larval lethality first filial generation, indicating essential during early developmental processes. In addition, accumulation abnormal endomitotic oocytes defects were parental animals. These findings suggest multiple phenotypes caused by disruption pH homeostasis due defective V-ATPase. summary, encoding C. elegans embryogenesis endocytosis.