Partial assembly of the yeast vacuolar H(+)-ATPase in mutants lacking one subunit of the enzyme

摘要: Partial assembly of the peripheral and integral membrane sectors yeast vacuolar H(+)-ATPase has been detected in mutants lacking one subunit enzyme. Assembled complexes could be immunoprecipitated from biosynthetically labeled wild-type cells using monoclonal antibodies specific for 69- 60-kDa subunits enzyme, assembled (V0) a antibody against 100-kDa subunits. Parallel immunoprecipitations mutant revealed different degrees depending on that was missing. Partially also soluble, cytoplasmic fraction following glycerol gradient fractionation. The results indicate (V1) sector can assemble independently. 69-, 60-, 27-kDa all appear to necessary any V1 occur, but these 32-kDa into complex absence 42-kDa subunit. implications structure are discussed.