Serum albumin uptake in isolated perfused renal tubules. Quantitative and electron microscope radioautographic studies in three anatomical segments of the rabbit nephron.

作者: James E. Bourdeau , Frank A. Carone , Charles E. Ganote

DOI: 10.1083/JCB.54.2.382

关键词: Convoluted tubuleSerum albuminAlbuminElectron microscopeVesicleKidneyNephronBiologyBiochemistryBiophysicsAcid phosphatase

摘要: Proximal convoluted, proximal straight, and cortical collecting tubular segments isolated from rabbit kidney were perfused with I 125-labeled serum albumin (RSA-I 125) in ultrafiltrate of for up to 3 hr After perfusion, the fixed glutaraldehyde, embedded Epon, either counted a gamma spectrometer quantitate protein accumulation or analyzed by electron microscope radioautography sequentially localize radioactivity convoluted straight accumulate RSA-I 125 nearly linearly as function time whereas do not measurable amounts protein. The rate tubule is 2 6 times great that tubule. Electron demonstrated taken via small apical vesicles invaginations, released into large cytoplasmic vacuoles, finally concentrated membrane-bounded structures, some which are acid phosphatase positive These results show absorbed tubules may be degraded intracellularly within lysosomes. In addition, less was located at all over lateral intercellular basilar labyrinthine spaces, suggesting labeled and/or its breakdown products transported across peritubular cell membrane.

参考文章(20)
Joseph L. Izzo, William F. Bale, Mary Jane Izzo, Angela Roncone, High Specific Activity Labeling of Insulin with 131I Journal of Biological Chemistry. ,vol. 239, pp. 3743- 3748 ,(1964) , 10.1016/S0021-9258(18)91198-1
Irving L. Spar, Ruth L. Goodland, William F. Bale, Letitia E. Mutschler, Ralph W. Helmkamp, High specific activity iodination of gamma-globulin with iodine-131 monochloride. Cancer Research. ,vol. 20, pp. 1495- 1500 ,(1960)
Arvid B. Maunsbach, Absorption of I125-labeled homologous albumin by rat kidney proximal tubule cells Journal of Ultrastructure Research. ,vol. 15, pp. 197- 241 ,(1966) , 10.1016/S0022-5320(66)80108-9
A. Lewis Farr, Oliver H. Lowry, Rose J. Randall, Nira J. Rosebrough, Protein Measurement with the Folin Phenol Reagent Journal of Biological Chemistry. ,vol. 193, pp. 265- 275 ,(1951)
Harry B. Neustein, Hemoglobin absorption in the proximal tubules of the kidney in the rabbit Journal of Ultrastructure Research. ,vol. 17, pp. 565- 587 ,(1967) , 10.1016/S0022-5320(67)80141-2
C. J. Bentzel, D. R. Tourville, B. Parsa, T. B. Tomasi, Bidirectional transport of horseradish peroxidase in proximal tubule of Necturus kidney. Journal of Cell Biology. ,vol. 48, pp. 197- 202 ,(1971) , 10.1083/JCB.48.1.197
Richard Rodewald, SELECTIVE ANTIBODY TRANSPORT IN THE PROXIMAL SMALL INTESTINE OF THE NEONATAL RAT Journal of Cell Biology. ,vol. 45, pp. 635- 640 ,(1970) , 10.1083/JCB.45.3.635
Charles E. Ganote, Jared J. Grantham, Harold L. Moses, Maurice B. Burg, Jack Orloff, ULTRASTRUCTURAL STUDIES OF VASOPRESSIN EFFECT ON ISOLATED PERFUSED RENAL COLLECTING TUBULES OF THE RABBIT Journal of Cell Biology. ,vol. 36, pp. 355- 367 ,(1968) , 10.1083/JCB.36.2.355