作者: James E. Bourdeau , Frank A. Carone , Charles E. Ganote
DOI: 10.1083/JCB.54.2.382
关键词: Convoluted tubule 、 Serum albumin 、 Albumin 、 Electron microscope 、 Vesicle 、 Kidney 、 Nephron 、 Biology 、 Biochemistry 、 Biophysics 、 Acid phosphatase
摘要: Proximal convoluted, proximal straight, and cortical collecting tubular segments isolated from rabbit kidney were perfused with I 125-labeled serum albumin (RSA-I 125) in ultrafiltrate of for up to 3 hr After perfusion, the fixed glutaraldehyde, embedded Epon, either counted a gamma spectrometer quantitate protein accumulation or analyzed by electron microscope radioautography sequentially localize radioactivity convoluted straight accumulate RSA-I 125 nearly linearly as function time whereas do not measurable amounts protein. The rate tubule is 2 6 times great that tubule. Electron demonstrated taken via small apical vesicles invaginations, released into large cytoplasmic vacuoles, finally concentrated membrane-bounded structures, some which are acid phosphatase positive These results show absorbed tubules may be degraded intracellularly within lysosomes. In addition, less was located at all over lateral intercellular basilar labyrinthine spaces, suggesting labeled and/or its breakdown products transported across peritubular cell membrane.