High-performance liquid chromatographic separation of iodoamino acids for tracer turnover studies of thyroid hormones in vivo.

摘要: Abstract A reversed-phase high-performance liquid chromatographic technique was developed to separate radioiodinated thyroxine (T4), 3,5,3′-triiodothyronine (T3), 3,3′,5′-triiodothyronine (rT3) two diiodothyronines 3,3′-T2 3′,5′-T2), in extracts from either serum or urine. Chromatography performed with 10-μm C18 silica gel, packed a glass column 3 × 300 mm); the mobile phase methanol—water (55:45) adjusted pH H3PO4, at flow-rate of 1.2 ml/min and pressure 2800 p.s.i. The results demonstrate ability system yield clear-cut separation iodothyronines involved vivo turnover studies, i.e., T4, T3, rT3, T2 compounds together.