The electrophoretic mobility of tripeptides as a function of pH and ionic strength: comparison with iontophoretic flux data.
作者： Barbara VanOrman Huff , Gary G. Liversidge , Gregory L. McIntire
关键词: Ionic strength 、 Tripeptide 、 Capillary electrophoresis 、 Iontophoresis 、 Flux (metabolism) 、 Chemistry 、 Electrophoresis 、 Chromatography 、 Molecule 、 Function (biology)
摘要: Capillary electrophoresis (CE) is an extremely efficient separations tool which can also be used to determine fundamental molecular parameters, e.g., the electrophoretic mobility of a molecule. We have studied changes in CE estimated thyrotropin releasing hormone (TRH) as function pH and ionic strength. Further, we estimate mobilities two synthetic analogs TRH examine behavior positive (basic) negative (acidic) peptides under conditions this work. These data were then compared with literature values iontophoretic flux these molecules similar formulation conditions. Our results suggest that could potentially assist optimization for delivery peptides.
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Michael J. Pikal, Saroj Shah, Transport mechanisms in iontophoresis. II. Electroosmotic flow and transference number measurements for hairless mouse skin. Pharmaceutical Research. ,vol. 7, pp. 213- 221 ,(1990) , 10.1023/A:1015857608850
Michael J. Pikal, Transport mechanisms in iontophoresis. I. A theoretical model for the effect of electroosmotic flow on flux enhancement in transdermal iontophoresis. Pharmaceutical Research. ,vol. 7, pp. 118- 126 ,(1990) , 10.1023/A:1015816532532
Michael J. Pikal, Saroj Shah, Transport mechanisms in iontophoresis. III. An experimental study of the contributions of electroosmotic flow and permeability change in transport of low and high molecular weight solutes. Pharmaceutical Research. ,vol. 7, pp. 222- 229 ,(1990) , 10.1023/A:1015809725688
Lewis E. Gibson, Iontophoretic sweat test for cystic fibrosis: technical details. Pediatrics. ,vol. 39, pp. 465- 465 ,(1967)
Lewis E. Gibson, Robert E. Cooke, A TEST FOR CONCENTRATION OF ELECTROLYTES IN SWEAT IN CYSTIC FIBROSIS OF THE PANCREAS UTILIZING PILOCARPINE BY IONTOPHORESIS Pediatrics. ,vol. 23, pp. 545- 549 ,(1959)
Randy M. McCormick, Capillary zone electrophoretic separation of peptides and proteins using low pH buffers in modified silica capillaries. Analytical Chemistry. ,vol. 60, pp. 2322- 2328 ,(1988) , 10.1021/AC00172A003
A.S. Cohen, B.L. Karger, High-performance sodium dodecyl sulfate polyacrylamide gel capillary electrophoresis of peptides and proteins. Journal of Chromatography A. ,vol. 397, pp. 409- 417 ,(1987) , 10.1016/S0021-9673(01)85026-3
V. Srinivasan, Muh-Hwan Su, W.I. Higuchi, C.R. Behl, Iontophoresis of polypeptides: effect of ethanol pretreatment of human skin. Journal of Pharmaceutical Sciences. ,vol. 79, pp. 588- 591 ,(1990) , 10.1002/JPS.2600790708
A. S. Cohen, A. Paulus, B. L. Karger, High-performance capillary electrophoresis using open tubes and gels Chromatographia. ,vol. 24, pp. 15- 24 ,(1987) , 10.1007/BF02688463
A.S. Cohen, D. Najarian, John A. Smith, B.L. Karger, Rapid separation of DNA restriction fragments using capillary electrophoresis. Journal of Chromatography A. ,vol. 458, pp. 323- 333 ,(1988) , 10.1016/S0021-9673(00)90576-4