Optimized synthesis of 100 nm diameter magnetoliposomes with high content of maghemite particles and high MRI effect.

摘要: Magnetoliposomes are liposomes surrounding an iron oxide core, which used as contrast enhancing agents in magnetic resonance imaging (MRI). One method for producing magnetoliposomes consists of hydration a lipid film with citrate-coated particles followed by extrusion. Two parameters major importance vivo applications magnetoliposomes, namely their size, must be small, optimally around 100 nm diameter, order to ensure prolonged circulation the bloodstream, and content, maximal generating high MRI effect. We studied formation passive encapsulation maghemite (γ-Fe2O3) particle suspensions varying concentrations, objective small size content. The ratio was determine content after successive purification steps cryo-TEM characterize homogeneity efficiency purification. clusters found critical obtaining smaller than 200 nm. were able reproducibly synthesize diameter –up 77 per liposome (5.6 moles mole lipid) – r2 relaxivity up 320 m m−1 . s−1. present improved characteristics compared previous reports. Future research will focus on using these drug delivery systems diagnostics or therapeutics applications. Copyright © 2011 John Wiley & Sons, Ltd.