The mechanism of cerebral acupuncture affects neuronal cell apoptosis via BrdU and Nestin in hypoxic-ischemic brain-damaged immature rats
作者： Qing Yuan , Xinger Li , Mingyin Lai , Fei Chen , Rong Zhao
DOI: 10.18088/EJBMR.2.2.2016.PP30-36
关键词: Neural stem cell 、 Nestin 、 Brain damage 、 Cerebral cortex 、 Acupuncture 、 Apoptosis 、 Brain ischemia 、 Medicine 、 Andrology 、 Immunology 、 TUNEL assay
摘要: Objective: In this study, the effects of scalp acupuncture on number apoptotic cells and indexes were deterrmined in rats with intrauterine hypoxic-ischemic brain damage(HIBD), inhibitory mechanism mitochondrial pathway, mediated apoptosis repair ischemia hypoxia was examined. Methods: A clip applied to uterine artery for 5 min before a cesarean section performed rat model. The divided into normal group (n = 24), model 24). Rats treated continuous head beginning 28th day after birth. sacrificed at days 35 , 42 49 samples analyzed by TUNEL staining obtained from cerebral cortex. each 8) determined compared. An immunohistochemical assay cortex examine BrdU Nestin expression. Results: Compared group，the significantly higher increased over time, (P < 0.05). expression than group, lower decrease time. decreased, while increased. Conclusion: Acupuncture can reduce inhibited neural stem cells, promoting injured neurons via cells.
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