Physical exercise improves brain cortex and cerebellum mitochondrial bioenergetics and alters apoptotic, dynamic and auto(mito)phagy markers.

摘要: We here investigate the effects of two exercise modalities (endurance treadmill training-TM and voluntary free-wheel activity-FW) on brain cortex cerebellum mitochondrial bioenergetics, permeability transition pore (mPTP), oxidative stress, as well proteins involved in biogenesis, apoptosis, quality control. Eighteen male rats were assigned to sedentary-SED, TM FW groups. Behavioral alterations ex vivo function endpoints assessed. Proteins phosphorylation (OXPHOS, including adenine nucleotide translocator), stress markers regulatory (SIRT3, p66shc, UCP2, carbonyls, MDA, -SH, aconitase, Mn-SOD), biogenesis (PGC1α, TFAM) evaluated. Apoptotic signaling was measured through quantifying caspase 3, 8 9-like activities, Bax, Bcl2, CypD, cofilin expression. Mitochondrial dynamics (Mfn1/2, OPA1 DRP1) auto(mito)phagy (LC3II, Beclin1, Pink1, Parkin, p62)-related also by Western blotting. Only group showed increased spontaneous alternation exploratory activity. Both regimens improved respiratory activity, OXPHOS complexes I, III V subunits both subareas decreased markers. Increased resistance mPTP apoptotic observed from Also, expression autophagy fusion, simultaneous with fission-related protein DRP1. In conclusion, physical improves function, decreasing related It is possible that favorable induced contributed plasticity leading a more robust phenotype.