The Cryoprotective Effect on Frozen-thawed Boar Semen of Egg Yolk Low Density Lipoproteins

摘要: In order to protect the spermatozoa against cold shock, hen egg yolk is widely used as a cryoprotective agent in semen freezing extenders for domestic animals. The protective action of largely presumed be due low density lipoproteins (LDL). effects LDL on sperm quality bull and northern pike (Esox lucius) after freezing-thawing have been reported, but no study has made evaluate effect boar motility other characteristics. experiment was carried out investigate 0.25 ml straws. aim cryopreserved presence LDL. Motility analyzed compared with Tris-citric acid-glucose (TCG) acid-fructose (TCF), two basic containing yolk. Similarly, acrosome plasma membrane integrity were also evaluated TCG TCF. Analysis freeze-thaw showed that motility, improved extender, highest post-thaw obtained 9% (w/v). Consequently, optimum concentration extender 9%. It suggested addition important protection during thawing. percentage motile significantly higher than TCF 54.4% versus 30.4% 30.1% (p<0.05), respectively. at 70.3% 50.5% respectively frozen 37.8% 30.3% 36.4% 29.9%, (p<0.05),. conclusion, we propose extracted from could media better efficiency quality, allowing process. Furthermore, found enhanced (Asian-Aust. J. Anim. Sci. 2006.