Determination of plasma membrane characteristics of boar spermatozoa and their relevance to cryopreservation.
作者： J. A. Gilmore , J. Liu , A. T. Peter , J. K. Critser
DOI: 10.1095/BIOLREPROD58.1.28
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摘要: The osmotic tolerance limits for boar spermatozoa were determined at 22°C. These cells can swell to within 1.02 times and shrink 0.97 their isosmotic volume maintain > 70% motility. In the presence of an extender, 1.1 Plasma membrane permeability coefficients in 1 M dimethyl sulfoxide (DMSO), glycerol, 2 ethylene glycol (EG) 22 0 C. Hydraulic conductivity (Lp) was estimated be 0.120 0.016 (mean + SEM), 0.138 ± 0.006, 0.204 0.021 lim/min/atm DMSO, EG, respectively, Solute (PcPA) 0.930 0.118, 0.481 0.045, 1.98 0.106 X 10-3 cm/min, respectively. Subsequent experiments performed 8C 0°C. Activation energies calculated L glycerol EG 7.20 11.51 Kcal/mol, activation PCPA 4.06 7.48 Kcal/mol permeability, characteristics used calculate flux during addition removal cryoprotectant agents as well cooling warming. addition, potential intracellular ice formation warming calculated.
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