Isothermal DNA amplification with functionalized graphene and nanoparticle assisted electroanalysis for rapid detection of Johne’s disease

摘要: Abstract Johne’s disease (JD), which is caused by Mycobacterium avium subspecies paratuberculosis (MAP), a bacterial infection of the intestinal tract ruminants. JD cause significant economic and animal loss throughout world. Sensitive, selective, on-site detection MAP in clinical samples has always been problem. This study outlines loop-mediated isothermal DNA amplification (LAMP) electrochemical analysis-based point-of-care (POC) methodology for MAP. LAMP contributed to selective DNA, analysis assisted rapid sensitive products. A graphene tetracycline (TET)-functionalized screen printed carbon electrode was used magnesium pyrophosphate (Mg2P2O7) produced during LAMP. The Mg2P2O7 obtained from sandwiched on between TET zirconium dioxide nanoparticles (ZrO2). complexation triggered an change that monitored using techniques. mechanism functionalized electrodes were characterized several microscopic, spectroscopic, optimized biosensor employed detect range concentrations. Using impedance spectroscopy, limit 6.37 aM (20 fg/μL) with 6.37 pM (20 ng/μL) obtained. application also assessed fecal samples. could easily presence bovine showed good co-relation other conventional Therefore, developed potential be POC animals.