An impedimetric biosensor to test neat serum for dengue diagnosis

摘要: Abstract NS1, a non-structural dengue protein, has been used as biomarker for the detection of viremia in diagnosis. In this work, an impedimetric label-free immunosensor based on anti-NS1 modified gold electrode was developed. To immobilize anti-NS1, mixed self-assembled monolayer consisting 11-mercaptoundecanoic acid (for covalent attachment) and 6-mercaptohexanol (as spacer) prepared. The impedance spectra were recorded presence redox probe ([Fe(CN)6]3−/4−) to monitor changes charge-transfer resistance associated with target binding both PBS neat serum. A calibration curve constructed order obtain analytical parameters. NS1 diluted final concentrations linear range 0.01–2.00 μg mL−1 sensitivity 14.1 percentage decade−1 (R ∼ 0.99) limit (LOD) 3 ng mL−1. 0.01–1.00 μg mL−1 serum also obtained (R ∼ 0.98) 10.4 percentage decade−1 LOD 30 ng mL−1. results open new insights into potential use biosensor point-of-care bedside applications