Hyaluronan synthesis by developing cortical neurons in vitro.

摘要: Hyaluronan is a linear glycosaminoglycan that forms the backbone of perineuronal nets around neurons in cerebral cortex. However, it remains controversial whether are capable independent hyaluronan synthesis. Herein, we examined expression and synthases (HASs) throughout cortical neuron development vitro. Enriched cultures were established from E16 rats. Neurons collected at days vitro (DIV) 0 (4 h), 1, 3, 7, 14, 21 for qPCR or immunocytochemistry. In relative absence glia, exhibited HAS1–3 mRNA all time-points. By immunocytochemistry, puncta HAS2–3 protein located on neuronal cell bodies, neurites, lamellipodia/growth cones as early 4 h culture. As matured, was also detected dendrites, filopodia, axons, synapses. Percentages hyaluronan-positive increased with culture time to ~93% by DIV21, while only half DIV21 expressed net marker Wisteria floribunda agglutinin. These data clearly demonstrate can independently synthesise maturational stages, production not limited expressing nets. The specific structural localisation suggests potential roles function.