Transient inflammatory response induced by apoptotic cells is an important mediator of melanoma cell engraftment and growth

摘要: Two murine melanoma cell lines, Tm1 and Tm5, were derived from a nontumorigenic lineage of pigmented melanocytes, melan-a. Both Tm5 are invariably tumorigenic in syngeneic mice when inoculated s.c. doses higher than 104 cells; 103 or fewer cells rarely give rise to tumors. We demonstrate that subtumorigenic inocula (103) as well known line (B16F10) develop vigorously growing tumor grafts only coinoculated with apoptotic, but not necrotic cells. The presence apoptotic correlates transient inflammatory infiltrate, composed mainly neutrophils macrophages. Kinin B1 receptor–deficient mice, which have impaired transmigration inflamed tissues, had significant growth inhibition coinjected Using the same model, take athymic was similar seen wild-type suggesting T cell–dependent response is necessary promote survival Taken together, our results describe how engraftment can be profoundly affected by microenvironmental alterations Disrupting delicate balance between leukocyte infiltration may provide potentially important insights for understanding interfering viability during treatment either γ-radiation apoptosis-inducing drugs. © 2004 Wiley-Liss, Inc.