A model of intrauterine infection and preterm delivery in mice

摘要: OBJECTIVE: Our purpose was to determine whether intrauterine bacterial inoculation leads preterm delivery in mice. STUDY DESIGN: Fifty-four female CD-1 mice at 75% of the length gestational period (14.5 days) received either an inoculum 2 10 × 103Escherichia coli (n = 33), intraperitoneal 7), or injection a sterile solution 14). RESULTS: Delivery within 48 hours surgery occurred 91% after bacteria, 0% and 7% (p < 0.001). Intrauterine produced systemic infection (i.e., recovery organisms from culture heart) 50% animals post partum. Intraperitoneal bacteria saline injections resulted rates 20% 0%, respectively, surgery. Five seven injected with into uterus had histologic evidence metritis, mild all cases. induction ribonucleic acid transcripts for tumor necrosis factor-α, interleukin-1α, interleukin-1β, cyclooxygenase-2. CONCLUSIONS: Escherichia local factors known be involved human labor infection. The observation that does not result suggests this model is product (uterine) stimulus.