Ascitic Fluid from Human Ovarian Cancer Patients Contains Growth Factors Necessary for Intraperitoneal Growth of Human Ovarian Adenocarcinoma Cells

摘要: Human ovarian cancer, the leading cause of death from gynecologic malignancy, tends to remain localized peritoneal cavity until late in disease. In established disease, ascitic fluid accumulates cavity. We have previously demonstrated that this is a potent source vitro mitogenic activity including at least one unique growth factor. now report human adenocarcinoma line, HEY, can be induced grow intraperitoneally immunodeficient nude mice presence (23/28 mice), but not absence (0/21 mice) cancer patients. Ascitic patients with benign disease did similar effects on intraperitoneal HEY cells (1/15 mice). Once tumors were by injections exogenous fluid, they could progress additional fluid. The eventually developed which contained factor activity, suggesting produced autologous factors. This mouse model provides system study action factors tumor vivo and evaluate preclinically, therapeutic approaches designed counteract these