A Transcriptomic Analysis of Echinococcus granulosus Larval Stages: Implications for Parasite Biology and Host Adaptation

摘要: Background: The cestode Echinococcus granulosus - the agent of cystic echinococcosis, a zoonosis affecting humans and domestic animals worldwide is an excellent model for study host-parasite cross-talk that interfaces with two mammalian hosts. To develop molecular analysis these interactions, we carried out EST survey E. larval stages. We report salient features this focus on genes reflecting physiological adaptations different parasite Methodology/Principal Findings: generated ,10,000 ESTs from sets full-length enriched libraries (derived oligo-capped trans-spliced cDNAs) prepared three materials: hydatid cyst wall, worms (protoscoleces), pepsin/H + -activated protoscoleces. were clustered into 2700 distinct gene products. In context biology granulosus, our analyses reveal: (i) diverse group abundant long non-protein coding transcripts showing homology to middle repetitive element (EgBRep) could either be active species or represent precursors small RNAs (like piRNAs); (ii) up-regulation fermentative pathways in tissue wall; (iii) highly expressed thiol- selenol-dependent antioxidant enzyme targets thioredoxin glutathione reductase, functional hub redox metabolism parasitic flatworms; (iv) candidate apomucins external layer tissuedwelling cyst, mucin-rich structure critical survival intermediate host; (v) set tetraspanins, protein family appears have expanded lineage; (vi) platyhelminth-specific products may offer novel pan-platyhelminth drug development. Conclusions/Significance: This has greatly increased quality quantity information constitutes valuable resource prediction genome further genomic proteomic focused cestodes platyhelminths.