Forced vortex assisted liquid phase microextraction for preconcentration and spectrophotometric determination of mefenamic acid in biological samples

摘要: Abstract A new microextraction approach termed forced vortex assisted liquid phase (FVA-LPME) has been developed and optimized for spectrophotometric determination of mefenamic acid (MFA) as a model compound. FVA-LPME is based on the formation rotational organic within aqueous subsequent partition target analyte between two phases. Rotation fine layer solid body accelerates mass transfer improves extraction efficiency. MFA extracted into by procedure subsequently derivatized iodine to form charge complex detection. response surface methodology (RSM) central composite chemometrics design was used multivariate optimization effects three different parameters influencing Under optimal conditions, calibration curve linear in range 10–900 ng mL −1 with R 2 0.998. The repeatability reproducibility (RSD%) 200 ng mL were 2.6% 3.3%, respectively limit detection (S/N = 3) estimated be 3.1 ng mL . results demonstrated that method an accurate, inexpensive, rapid reliable sample pretreatment gives very good enrichment factor extracting determining biological samples.