Wogonoside inhibits angiogenesis in breast cancer via suppressing Wnt/β‐catenin pathway

摘要: Wogonoside, a main flavonoid component derived from the root of Scutellaria baicalensis Georgi, has been reported to have anti-angiogenesis and anti-leukemia activities. However, whether it can inhibit tumor angiogenesis is unclear. In this study, we investigate inhibitory effect wogonoside on in breast cancer its underlying mechanisms. ELISA assay shows that (25, 50, 100 µM) decreases secretion VEGF MCF-7 cells by 30.0%, 35.4%, 40.1%, respectively. We find inhibits induced conditioned media vitro vivo migration, tube formation, rat aortic ring, chicken chorioallantoic membrane (CAM) assay. Meanwhile, growth xenografts nude mice. The reduction weight be found both (80 mg/kg) bevacizumab (20 mg/kg) treated group, inhibition rate 42.1% 48.7%, addition, mechanistic studies demonstrate suppresses activation Wnt/β-catenin pathway cells. Wogonoside (100 µM) intracellular level Wnt3a, increases expression GSK-3β, AXIN, promotes phosphorylation β-catenin for proteasome degradation significantly. Furthermore, nuclear accumulation DNA-binding activity β-catenin/TCF/Lef complex are inhibited 49.2% 28.7%, respectively, when with 100 µM wogonoside. Taken together, our findings potential inhibitor developed as therapeutic agent cancer. © 2015 Wiley Periodicals, Inc.