Bacterial colonization of amniotic fluid in the presence of ruptured membranes

摘要: Amniotic fluid (AF) was collected from 37 selected patients by amniocentesis, aspiration through a pressure catheter, or at the time of cesarean section. The unspun AF examined directly Gram stain for bacteria and white blood cells (WBC) cultured. Thirteen cultures were positive, defined as growth on primary plating media which corresponded to greater than 10(2) colony-forming units (CFU) per milliliter. Almost equal numbers aerobic anaerobic isolated. presence bacteria, but not WBC, correlated significantly with positive culture, indicated that microscopic examination would usually predict culture result. Growth CFU/ml associated clinical chorioamnionitis, colonization also observed in five afebrile patients, four whom premature labor. In delivered section, each postpartum endometritis.