?Cytogenetic? studies of spermatids of mice carrying Cattanach's translocation by flow cytometry
作者： Marvin L. Meistrich , Wolfgang G�hde , R. Allen White , Jill L. Longtin
DOI: 10.1007/BF00292269
关键词: Gene 、 Developmental biology 、 DNA 、 Biology 、 Chromosomal translocation 、 Ploidy 、 Molecular biology 、 Haploid genome 、 Flow cytometry 、 Spermatogenesis 、 Genetics(clinical) 、 Genetics
摘要: The DNA content of spermatids mice carrying Cattanach's translocation has been measured with high precision by flow cytometry. observation that the two peaks in haploid region histograms represent X-and Y-bearing was tested and confirmed. Using cytometry, difference between X Y chromosomes these to be 5.2±0.1% total genome as compared 3.4±0.1% normal mice. These results demonstrated cytometry for cytogenetic studies. Additional information on spermatogenesis bearing obtained showed a gradual loss cells during those balanced form translocation.
springer.com
sci-hub.se 参考文章(17)
H Hoehn, J Callis, Flow-fluorometric diagnosis of euploid and aneuploid human lymphocytes. American Journal of Human Genetics. ,vol. 28, pp. 577- 584 ,(1976)
Marvin L. Meistrich, Chapter 2 Separation of Spermatogenic Cells and Nuclei from Rodent Testes Methods in Cell Biology. ,vol. 15, pp. 15- 54 ,(1977) , 10.1016/S0091-679X(08)60207-1
Eva M. Eicher, X-Autosome Translocations in The Mouse: Total Inactivation Versus Partial Inactivation Of The X Chromosome Advances in Genetics. ,vol. 15, pp. 175- 259 ,(1970) , 10.1016/S0065-2660(08)60074-7
T H Roderick, Committee on Standard Genetic Nomenclature for Mice: Standard karyotype of the mouse, Mus musculus. pp. 69- ,(1972)
Marvin L. Meistrich, Suzanne Lake, Lloyd L. Steinmetz, Barton L. Gledhill, Increased variability in nuclear DNA content of testis cells and spermatozoa from mice with irregular meiotic segregation. Mutation Research. ,vol. 49, pp. 397- 405 ,(1978) , 10.1016/0027-5107(78)90110-0
J�rgen Zante, Johannes Schumann, Wolfgang G�hde, Ursula Hacker, DNA-fluorometry of mammalian sperm Histochemistry and Cell Biology. ,vol. 54, pp. 1- 7 ,(1977) , 10.1007/BF00493324
Bruce M. Cattanach, A chemically-induced variegated-type position effect in the mouse. Zeitschrift fur Vererbungslehre. ,vol. 92, pp. 165- 182 ,(1961) , 10.1007/BF00890283
MARVIN L. MEISTRICH, WOLFGANG GÖHDE, R. ALLEN WHITE, JOHANNES SCHUMANN, Resolution of X and Y spermatids by pulse cytophotometry. Nature. ,vol. 274, pp. 821- 823 ,(1978) , 10.1038/274821A0
M A Van Dilla, B L Gledhill, S Lake, P N Dean, J W Gray, V Kachel, B Barlogie, W Göhde, Measurement of mammalian sperm deoxyribonucleic acid by flow cytometry. Problems and approaches. Journal of Histochemistry and Cytochemistry. ,vol. 25, pp. 763- 773 ,(1977) , 10.1177/25.7.70455
M.L. Meistrich, W.R. Bruce, Y. Clermont, Cellular composition of fractions of mouse testis cells following velocity sedimentation separation Experimental Cell Research. ,vol. 79, pp. 213- 227 ,(1973) , 10.1016/0014-4827(73)90507-7