Detection of aneuploid human sperm by fluorescence in situ hybridization : Evidence for a donor difference in frequency of sperm disomic for chromosomes 1 and Y

摘要: Fluorescence in situ hybridization with repetitive-sequence DNA probes was used to detect human sperm disomic for chromosomes 1 and Y three healthy men. Data on these same men had been obtained previously, using the human-sperm/hamster-egg cytogenetic technique, providing a reference validating measurements. Air-dried smears were prepared from semen samples treated DTT lithium diiodosalicylate expand chromatin. Hybridization fluorescently tagged (pUC177) or (pY3.4) yielded average frequencies of two fluorescent domains 14.2[+-]2.4/10,000 5.6[+-]1.6/10,000 sperm, respectively. These did not differ statistically hyperploidy observed hamster technique. In addition, one donor elevated [approximately]2.5-fold above those other donors, both (P = .045) .01), consistent trend found The authors conclude that fluorescence provides valid promising measure frequency sperm. 43 refs., fig., 4 tabs.