Fluorescence In Situ Hybridization with Chromosome Paint Probes: A Novel Approach To Assess Aneuploidy in Human Sperm Nuclei

摘要: Purpose: Fluorescence in situ hybridization (FISH) using whole-chromosome paint probes was performed to evaluate disomy and diploidy frequency for chromosomes 1, 18, 19, 22 human sperm nuclei. Methods: Ten subjects of proven fertility normal spermatic parameters were included the study. A dual-color FISH method carried out. Results: total 157,896 spermatozoa scored. The mean frequencies disomic 0.22% (range, 0.19 0.28%), 0.24% 0.14 0.37%), 0.17 0.30%), 0.25% 0.21 0.29%), respectively. 0.14% 0.09 0.18%). No interindividual interchromosomal variations aneuploidy observed between different subjects. Conclusions: with provides a novel efficient approach assessment nuclei.